Caralluma umbellata Haw. protects liver against paracetamol toxicity and inhibits CYP2E1
نویسندگان
چکیده
Introduction: Paracetamol is a potent hepatotoxin and may cause severe acute hepatocellular injury. The present study was intended to assess the hepatoprotective potential of Caralluma umbellata Haw. (Asclepiadaceae) against paracetamol induced hepatotoxicity in vitro and in vivo experimental models. Methods: Preliminary analysis for antioxidant and hepatoprotective property was evaluated for methanolic (MCU), aqueous (ACU) and hydro-methanolic (HCU) extracts of C. umbellata using in vitro cell free antioxidant such as DPPH, ABTS, nitric oxide, lipid peroxidation models and cell based hepatoprotective study using BRL3A cells. In vivo, hepatoprotective activity was studied in paracetamol treated male Wistar albino rats. Furthermore, molecular mechanism behind the protective effect of MCU was explored by polymerase chain reaction (PCR) technique by cytochrome P450 (CYP) CYP2E1 expression. Results: C. umbellata extracts, especially MCU showed better antioxidant property. MCU offered significant dose dependent protection against paracetamol-induced hepatic damage in both in vitro and in vivo assays by improving all the biochemical findings towards the normal range. In mechanism based study, MCU showed significant down regulation (P < 0.05) of CYP2E1. These findings were in line with the hepatoprotective activity findings where MCU offered significant protection. Conclusion: In conclusion these findings suggest that MCU possess hepatoprotective activity. One of the possible mechanisms behind the protective effect of MCU is found to be the inhibition of CYP2E1 expression. Article Type: Original Article Article History: Received: 26 July 2017 Revised: 29 Aug. 2017 Accepted: 2 Oct. 2017 ePublished: 30 Oct. 2017
منابع مشابه
Anti-hyperglycemic activity of Caralluma umbellata Haw.
INTRODUCTION Hyperglycemia is a serious health problem prevailing in diabetes patients. Treatment for hyperglycemia by various oral anti-hyperglycemic drugs have associated with side effects, hence there is growing awareness towards the use of herbal products due to their efficacy, minimal side effects and relatively low costs. This study is designed to evaluate anti-hyperglycemic activity of C...
متن کاملThe liver-selective nitric oxide donor O2-vinyl 1-(pyrrolidin-1-yl)diazen-1-ium-1,2-diolate (V-PYRRO/NO) protects HepG2 cells against cytochrome P450 2E1-dependent toxicity.
HepG2 cells expressing CYP2E1 (E47 cells) are more susceptible to toxicity by arachidonic acid (AA) or after glutathione depletion with an inhibitor of glutamate-cysteine ligase, l-buthionine-(S,R)-sulfoximine (BSO), compared with control HepG2 cells (C34 cells). The ability of nitric oxide (NO) to protect against CYP2E1-dependent toxicity has not been evaluated. We therefore studied the abilit...
متن کاملParacetamol, alcohol and the liver.
It is claimed that chronic alcoholics are at increased risk of paracetamol (acetaminophen) hepatotoxicity not only following overdosage but also with its therapeutic use. Increased susceptibility is supposed to be due to induction of liver microsomal enzymes by ethanol with increased formation of the toxic metabolite of paracetamol. However, the clinical evidence in support of these claims is a...
متن کاملThe Liver-Selective Nitric Oxide Donor O-Vinyl 1-(pyrrolidin-1- yl)diazen-1-ium-1,2-diolate (V-PYRRO/NO) Protects HepG2 Cells against Cytochrome P450 2E1-Dependent Toxicity
HepG2 cells expressing CYP2E1 (E47 cells) are more susceptible to toxicity by arachidonic acid (AA) or after glutathione depletion with an inhibitor of glutamate-cysteine ligase, L-buthionine-(S,R)-sulfoximine (BSO), compared with control HepG2 cells (C34 cells). The ability of nitric oxide (NO) to protect against CYP2E1-dependent toxicity has not been evaluated. We therefore studied the abilit...
متن کاملFasting Reduces the Binding between Sugar and Protein; New Insights into Diabetic Complications
Fasting has numerous biological, physical and mental health advantages or that as some physicians cure their patients by prescribing fasting to them. Fasting protects people from many diseases such as cancer, cardiovascular diseases, and diabetes complications. The main health-promoting effects of fasting are increased production of neurotrophic factors, neuroendocrine activation, hormetic stre...
متن کامل